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Created by
user-9b773, last updated on Jul 08, 2016
2 minute read
The following protocols are no longer used or updated by our labs. Please contact Martin Wiedmann prior to use of any of these protocols.
8.1.1.2.2-Listeria PCRs by Gene Name
8.1.1.2.2.6-Multiplex PCR for inlH screening.pdf Archived Protocol [archived 07July2016]
8.1.1.2.2.7-Nested hly PCR for Listeria monocytogenes.pdf Archived Protocol [archived 07July2016]
8.1.1.2.2.8-Protocol for sequencing actA gene most polymorphic region.pdf Archived Protocol [archived 07July2016]
8.1.1.2.2.9-Protocol for Sequencing actA gene region with best discrimination.pdf Archived Protocol [archived 07July2016]
8.1.1.2.2.10-Protocol for Sequencing inlA gene region with the best discrimination.pdf Archived Protocol [archived 07July2016]
8.1.1.3-Salmonella PCR
8.1.1.3.1-fimA PCR and DNA sequencing of Salmonella enterica for 3gene MLST.pdf Archived Protocol [archived 07July2016]
8.1.1.3.2-manB PCR and DNA sequencing of Salmonella enterica for 3gene MLST.pdf Archived Protocol [archived 07July2016]
8.1.1.3.3-mdh PCR and DNA sequencing of Salmonella enterica for 3gene MLST.pdf Archived Protocol [archived 07July2016]
8.1.1.3.5-PCR detection of the presence or absence of antibiotic resistance genes in Salmonella.pdf Archived Protocol [archived 07July2016]
8.1.2-Phenotypic Characterization
8.1.2.1-Semi-Quantitative Hemolysis Assay for Listeria.pdf Archived Protocol [archived 07July12016]
8.1.3-RFLP
8.1.3.1-Polymerase Chain Reaction- Restriction Fragment Length Polymorphism.pdf Archived Protocol [archived 07July2016]
8.1.4-Southern Blot [section archived 07July 2016]
8.1.4.1-Southern Blotting by Electrophoretic Transfer and non radioactive Detection.pdf Archived Protocol
8.1.4.2-Southern Blotting.pdf Archived Protocol
8.1.4.3-SouthernBlot Hybridization.pdf Archived Protocol
8.2-DNA Labeling & Modification [section archived 07July2016]
8.2.1-3’ end labeling of oligonucleotides.pdf Archived Protocol
8.2.2-Kinasing and DIG labeling of LCR primer.pdf Archived Protocol
8.2.3-Poly-T tailing of oligonucleotides.pdf Archived Protocol
8.2.4-Protocol for Fluorescein labeling of oligonucleotides carrying an aminolink.pdf Archived Protocol
8.2.5-Protocol for Geneclean (USB).pdf Archived Protocol
8.3-DNA preparation, Purification, & Quantification
8.3.1-Chelex DNA extraction from blood.pdf Archived Protocol [archived on 07Jun2016]
8.3.2-Chelex DNA extraction from semen.pdf Archived Protocol [archived on 07Jun2016]
8.3.4-DNA Preparation from Listeria using Guanidinium Isothiocyanate.pdf Archived Protocol [archived on 07Jun2016]
8.3.7-PCR Purification Protocol for DNA Sequencing.pdf Archived Protocol [archived on 07July2016]
8.3.11-Single Stranded DNA preparation from M13.pdf Archived Protocol [archived on 07July2016]
8.6-Microarray
8.6.1-Analyzing Microarrays.pdf Archived Protocol [archived March 2016]
8.6.2-Creating Microarrays.pdf Archived Protocol [archived March 2016]
8.6.3-Labeling RNA with Cy3Cy5 and Hybridization for Microarrays.pdf Archived Protocol [archived March 2016]
8.6.4-Microarray Slide Blocking.pdf Archived Protocol [archived March 2016]
8.8-RNA Prep & Analysis
8.8.2-Isolation of RNA from Listeria monocytogenes infected mammalian cells.pdf Archived Protocol [archived April 2016]
8.8.3-Preparation of infected tissue culture cells for bacterial RNA collection.pdf Archived Protocol [archived April 2016]
8.8.4-Preperation of Listeria RNA.pdf Archived Protocol [archived April 2016]
8.8.6-RNA Prep with RNeasy Midi Kit instructions.pdf Archived Protocol [archived April 2016]
8.8.7-RNeasy kit total RNA isolation from Listeria monocytogenes with on-column DNase treatment.pdf Archived Protocol [archived April 2016]
8.8.8-RT-PCR for the detection of cytokine mRNA.pdf Archived Protocol [archived April 2016]
8.8.9-Synthesis of large amounts of RNA.pdf Archived Protocol [archived April 2016]