Molecular Biology ARCHIVE

The following protocols are no longer used or updated by our labs.  Please contact Martin Wiedmann prior to use of any of these protocols.

8.2-DNA Labeling & Modification [section archived 07July2016]

8.2.1-3’ end labeling of oligonucleotides.pdf

8.2.2-Kinasing and DIG labeling of LCR primer.pdf

8.2.3-Poly-T tailing of oligonucleotides.pdf

8.2.4-Protocol for Fluorescein labeling of oligonucleotides carrying an aminolink.pdf

8.2.5-Protocol for Geneclean (USB).pdf

8.3-DNA preparation, Purification, & Quantification

8.3.1-Chelex DNA extraction from blood.pdf Archived Protocol [archived on 07Jun2016] 

8.3.2-Chelex DNA extraction from semen.pdf Archived Protocol [archived on 07Jun2016] 

8.3.4-DNA Preparation from Listeria using Guanidinium Isothiocyanate.pdf Archived Protocol [archived on 07Jun2016] 

8.6-Microarray

8.6.1-Analyzing Microarrays.pdf Archived Protocol [archived March 2016]

8.6.2-Creating Microarrays.pdf Archived Protocol [archived March 2016] 

8.6.3-Labeling RNA with Cy3Cy5 and Hybridization for Microarrays.pdf Archived Protocol [archived March 2016] 

8.6.4-Microarray Slide Blocking.pdf Archived Protocol [archived March 2016] 

8.8-RNA Prep & Analysis

8.8.2-Isolation of RNA from Listeria monocytogenes infected mammalian cells.pdf Archived Protocol [archived April 2016] 

8.8.3-Preparation of infected tissue culture cells for bacterial RNA collection.pdf Archived Protocol [archived April 2016] 

8.8.4-Preperation of Listeria RNA.pdf Archived Protocol [archived April 2016] 

8.8.6-RNA Prep with RNeasy Midi Kit instructions.pdf Archived Protocol [archived April 2016] 

8.8.7-RNeasy kit total RNA isolation from Listeria monocytogenes with on-column DNase treatment.pdf Archived Protocol [archived April 2016] 

8.8.8-RT-PCR for the detection of cytokine mRNA.pdf Archived Protocol [archived April 2016] 

8.8.9-Synthesis of large amounts of RNA.pdf Archived Protocol [archived April 2016]