The following protocols are no longer used or updated by our labs.  Please contact Martin WiedmannNicole MartinAhmed GaballaSam Reichler, or Jordan Skeens to request access to these these protocols.


J774

10.3.1-J774 cells Culturing Conditions, Splitting and Freezing Protocol [archived 29April2020]

10.3.2-Labeling of Listerial Proteins J774 Cells.pdf [archived 8Aug16]

10.3.3-Quantitative Determination of L. monocytogenes Cell-to-Cell Spread in J774 Protocol [archived 29April2020]


L2 Mouse

10.4.1-DifQuik Staining of Listeria monocytogenes Growing Intercellularly in L2 Cells.pdf [archived 8Aug16]

10.4.2-Mouse L cells Culturing Conditions, Splitting and Freezing Protocol [archived 29April2020]

10.4.3-Passage of Mouse L Cells for Plaque Assay.pdf [archived 8Aug16]

10.4.4-Plaque Assay of Listeria monocytogenes Cytopathogenicity Using Mouse L Cells Protocol [archived 29April2020]

 

Other Cell Lines

10.5.1-Culturing Conditions, Splitting and Freezing of L929.pdf [archived 29April2020]

10.5.2-Growth of Primary Sheep Neurons in Cell Culture.pdf [archived 29April2020]

10.5.3-L. monocytogenes Infection of Primary Neuronal Cell Culture(DRG).pdf [archived 29April2020]

10.5.4-Lmono Intracellular Infection Assay in Fish Cells.pdf [archived 29April2020]

10.5.5-PC12 cells Culturing Conditions, and Freezing.pdf [archived 29April2020]

 

Media & Reagents

10.9.1-Preparation of 0.1% Trypsin-EDTA_22NOV10.pdf [archived 29April2020]