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Need to review with MW.

10.1 - Caco-

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10.1.1-Listeria monocytogenes Caco-2 cell invasion assay.pdf SOP Protocol [reviewed updated by ram524 on 28APR162020-06-01]

10.1.2-Maintenance of Caco-2 Cells_22NOV10.pdf Protocol [reviewed by ram524 on 28APR16 SOP [updated by ram524 2020-06-01]

10.1.3-Salmonella Caco-2 cell invasion assay.pdf SOP Protocol [reviewed updated by ram524 on 28APR162020-06-01]

10.2 - HeLa

10.2.1-Culturing Conditions, Splitting and Freezing of Hela.pdf Protocol [reviewed by ram524 on 20APR16]

10.2.2-Tissue culture assay to quantify bacterial adhesion to HeLa cells Protocol [reviewed mw16 8Aug2016]

10.3 - J774

[see archive]

10.4 - L2 Mouse

[see archive]

10.5 - Other Cell Lines

[see archive]

10.6 - HIEC-6

10.63.1-J774 cells Culturing Conditions, Splitting Passaging and Freezing HIEC-6 Protocol [reviewed mw16 8Aug2016ram524 28Jul2017]

10.36.32-Quantitative Determination of L. monocytogenes Cell-to-Cell Spread in J774 Protocol [reviewed mw16 8Aug2016]

L2 Mouse

10.4.2-Mouse L cells Culturing Conditions, Splitting and Freezing Protocol [reviewed mw16 8Aug2016]

10.4.4-Plaque Assay of Listeria monocytogenes Cytopathogenicity Using Mouse L Cells Protocol [reviewed mw16 8Aug2016]

Other Cell Lines

10.5.1-Culturing Conditions, Splitting and Freezing of L929.pdf

10.5.2-Growth of Primary Sheep Neurons in Cell Culture.pdf

10.5.3-L. monocytogenes Infection of Primary Neuronal Cell Culture(DRG).pdf

10.5.4-Lmono Intracellular Infection Assay in Fish Cells.pdf

10.5.5-PC12 cells Culturing Conditions, and Freezing.pdf

Media & Reagents

10.6.1-Preparation of 0.1% Trypsin-EDTA_22NOV10.pdf

Salmonella Invasion of HIEC-6 Cells SOP [created by ram524 2020-12-22]

10.7 - Flow Cytometry

10.7.1-DNA Content Analysis Protocol [reviewed ram524 28Jul2017]

10.8 - Staining and Microscopy

10.8.1-Immunofluorescent staining for gamma H2AX and 53BP1 Protocol [reviewed ram524 28Jul2017]

10.9 - Media & Reagents

[see archive]

 

 

Training Record

10.1.1-Listeria monocytogenes Caco-2 cell invasion assay.pdf Trainer                                                  
Trainee                                                  
Training Date                                                  
10.1.2-Maintenance of Caco-2 Cells_22NOV10.pdfTrainer                                                  
Trainee                                                  
Training Date                                                  
10.1.3-Salmonella Caco-2 cell invasion assay.pdfTrainer                                                  
Trainee                                                  
Training Date                                                  
10.2.1-Culturing Conditions, Splitting and Freezing of Hela.pdfTrainer                                                  
Trainee                                                  
Training Date                                                  
10.2.2-Tissue culture assay to quantify bacterial adhesion to HeLa cellsTrainer                                                  
Trainee                                                  
Training Date                                                  

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10.6.1-Passaging and Freezing HIEC-6

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Trainer                                                  
Trainee                                                  
Training Date                                                  
10.6.2-Infection of HIEC-6 cells with Salmonella

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Trainer                                                  
Trainee                                                  
Training Date                                                  

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10.7.1-DNA Content Analysis

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Trainer                                                  
Trainee                                                  
Training Date                                                  

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10.8.1-Immunofluorescent staining for gamma H2AX and 53BP1

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Trainer                                                  
Trainee                                                  
Training Date                                                  
 Trainer                                                  
Trainee                                                  
Training Date