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• Carefully remove the substrate from the sample holder.
• Place the substrate in a developer bath to wash away excess liquid 2PP resin.
• Wash away the developer.
• Transfer to IPA for at least 15 minutes.
• Gently blow-dry the sample (Optional- dependent on size and strength of your structure, use with extreme caution).
• Cure remaining liquid 2PP resin if a shell and scaffold structure was printed.

Clean the objective lens

• If an immersion objective was used, it is advised to clean the objective lens directly after printing. This is mandatory if a different The objective lenses must be cleaned after each user has finished using the tool. Multiple prints may be conducted during the same user session without the need to clean the objective, assuming the same 2PP resin will be used with the same objective, or if the print set is changed from oil immersion configuration to DiLL or vice versa.
• Cleaning the objective lens between jobs is recommended but is not strictly required if the configuration remains the same for printing subsequent structures.
Objectives should be cleaned at least once a week or after printing several structures
• for each print.
• In most cases, IPA is appropriate for lens cleaning.
  • Specific lenses have different cleaning requirements, as do different resins and immersion oils.
  • If you are unsure of the proper cleaning procedure for your use case, please contact the staff before printing.

10X Objective

• 1. Remove the objective and screw it into its holder
  2. Carefully wipe the metal portion of the objective with a lab wipe or swab, taking care not to touch the glass.
  3. Cover the lens with the clear top cover and move the lens to the development room.
  4. Place the lens on clean wipes where you will be cleaning - do no assume wipes left in the hood are clean.
  5. Carefully fold a wipe to about 1 to 1.5" wide and wrap around the body of the objective. Take note of the location of the vent hole by placing your thumb directly over it.
  6. Invert the lens over the waste container and funnel.
  7. Gently flow IPA over the objective using a wash bottle - be careful to dispense the IPA slowly and keep the tip of the bottle away from the nosepiece.
  8. With the lens still inverted, use the N2 blowgun and dry off the IPA with a low pressure stream of dry nitrogen
  9. Return the lens to an upright position and using a wipe or swab remove any resin which has flown onto the metal body of the nosepiece
  10. Repeat, typically 2-3 additional times, using IPA sparingly.
  11. Note, for use with GP-Silica, alternate cleaning methods must be used, as outlined in the section on GP-Silica

25X Objective

• 1. Remove the objective and screw it into its holder
  2. Carefully wipe the metal portion of the objective with a lab wipe or swab, taking care not to touch the glass.
  3. Check the objective body, and in particular, the adjustment ring for any residual resin and clean this with a lab wipe dampened with IPA.
  4. Cover the lens with the clear top cover and move the lens to the development room.
  5. Place the lens on clean wipes where you will be cleaning - do no assume wipes left in the hood are clean.
  6. Carefully fold a wipe to about 1 to 1.5" wide and wrap around the body of the objective.
  7. Follow steps 6-10 as above, being careful to prevent any IPA from coming into contact with the rubber gaskets and the adjustment ring in particular.

63X Objective

Follow cleaning for the 10X objective, omitting the need to cover a vent hole in step 5.

Leaving the printer

• Close NanoWrite. This makes sure that used print commands are set to the default values for the next user.
• Only switch off the laser and other printer components if the printer is not in use for a longer period of time.

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