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The peristaltic tubing sizes are rather arbitrary and are labeled by numbers: 13, 14, 16, 17, and 18 in increasing order of size. There is also 3-stop tubing available, which are color-coded based on their ID and must be used with 6-roller pump heads.
The table below gives you an idea of the flow rates that can be achieved by a peristaltic pump for different tubing sizes.
| Tubing Code | 13 | 14 | 16 | 17 | 18 | 3-Stop Tubing Yellow-Blue-Yellow | 3-Stop Tubing Purple-White-Purple | |
| RPM/ID (mm) | 0.8 | 1.6 | 3 | 6.3 | 8 | 1.52 | 2.79 | |
flow | 1 | 0.00100 | 0.00350 | 0.01333 | 0.04667 | 0.06333 | 0.0025 | 0.006167 |
| 100 | 0.10000 | 0.35000 | 1.33333 | 4.66667 | 6.33333 | 0.25 | 0.6166667 | |
| 600 | 0.60000 | 2.10000 | 8.00000 | 28.00000 | 38.00000 | - | - | |
| mL/rev | 0.06 | 0.21 | 0.8 | 2.8 | 3.8 | 0.025 | 0.06 |
From these charts, you can easily calculate a pump speed given a tubing number and flow rate. At the appropriate tubing number, divide the given flow rate by the flow rate at 1 rpm.
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