*** This version of Confluence is for testing only and contains a copy of content from June 29th 2026. No changes will be preserved. ***
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8.3.12-Small-scale phenol-chloroform extraction of chromosomal DNA from Listeria monocytogenes.pdf
8.3.13_-Genomic_DNA_Extraction_Qiagen_11_21_13.pdf
8.3.14_-HighThroughput_gDNA_Extraction_Qiagen_1_28_15.pdf
8.3.15_-Take3_NucleicAcid_Quantification_01_20_15.pdf
8.4-DNA Sequencing Analysis
8.4.1-Automated Sequence Editing SOP [reviewed by sjr267 03/23/2016]
8.5-DNA Recombination & Mutagenesis
8.45.1-Cloning of DNA Flanking Tn917-LTV3.pdf
8.45.2-Cloning of DNA Fragments.pdf
8.45.3-Cloning PCR products for Sequencing using pCR 2.1.pdf
8.45.4-Cloning Protocol for Transformation of E. coli DH5aF.pdf
8.45.5-Electroporation L mono.pdf
8.45.6-Electroporation of E coli.pdf
8.45.7-Electroporation of Mycobacterium paratuberculosis.pdf
8.45.8-General Cloning DNA.pdf
8.45.9-GUS promoter fusions in Listeria monocytogenes.pdf
8.45.10-Integration of pPL2 derivatives into L. monocytogenes 10403S.pdf
8.45.11-Listeria Genomic Alterations.pdf
8.45.12-Overview-Making a mutant in Listeria monocytogenes.pdf
8.45.13-Part II-Making a mutant in Listeria monocytogenes.pdf
8.45.14-Part III-Making a mutant in Listeria monocytogenes.pdf
8.45.15-Part IV-Making a mutant in Listeria monocytogenes.pdf
8.45.19-Procedure for cloning with pDH32.pdf
8.45.20-Whole Mutant- SoeingPCR for L. mono.pdf
8.
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6-Microarray
8.56.1-Analyzing Microarrays.pdf
8.56.2-Creating Microarrays.pdf
8.56.3-Labeling RNA with Cy3Cy5 and Hybridization for Microarrays.pdf
8.56.4-Microarray Slide Blocking.pdf
8.
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7-Protein Methods
8.67.2-Beta Galactosidase Assay.pdf
8.67.3-His tagged sigma factor protein purification protocol.pdf
8.
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8-RNA Prep & Analysis
8.78.1-DNase treatment and phenolchloroform extractions for high purity RNA.pdf
8.78.2-Isolation of RNA from Listeria monocytogenes infected mammalian cells.pdf
8.78.3-Preparation of infected tissue culture cells for bacterial RNA collection.pdf
8.78.4-Preperation of Listeria RNA.pdf
8.78.5-RNA extraction, clean-up and storage using Turbo DNase for TaqMan.pdf
8.78.6-RNA Prep with RNeasy Midi Kit instructions.pdf
8.78.7-RNeasy kit total RNA isolation from Listeria monocytogenes with on-column DNase treatment.pdf
8.78.8-RT-PCR for the detection of cytokine mRNA.pdf